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. 2004 Dec;78(23):13216–13231. doi: 10.1128/JVI.78.23.13216-13231.2004

FIG. 3.

FIG. 3.

Flow cytometry analysis of an SL3-3dm-induced mesenteric lymph node tumor (mouse no. 498-6) with the CD3+ immunophenotype after staining with antibodies to CD3-B220-CD11b (A), CD3-CD4-CD8a (B), B220-IgM-IgD (C), and B220-CD43-CD138 (D). The gated population is indicated in the top panel in each column. The tumor contains a large population of CD3+ T cells that do not coexpress CD11b (A) and show heterogeneous expression of CD4 and CD8 (B). Cells positive for B-cell surface markers B220, IgM, IgD, and CD138 are relatively rare (A, C, and D). Most cells are CD43+ (D). This tumor had TCRβ but not Ig(κ) clonally rearranged (data not shown), and the mouse was histologically diagnosed as having a small T-cell lymphoma. FSC, forward scatter; SSC, side scatter.