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. 2004 Dec;78(23):13216–13231. doi: 10.1128/JVI.78.23.13216-13231.2004

FIG. 6.

FIG. 6.

Southern blot analysis of genomic DNA extracted from SL3-3dm-induced tumors of distinct types. (A) DNA from SL3-3dm-induced T-cell tumors cleaved with HindIII and hybridized with the Ig(κ), TCRβ-J2, and IgH probes. Results are shown for 4 tumors from two different mice histologically diagnosed as having T-cell lymphoblastic lymphomas. Lanes: 1 and 2, spleen and lymph node tumors of mouse no. 499-8; 3 and 4, spleen and lymph node tumors of mouse no. 499-12. Black bars indicate the positions of the unrearranged genomic bands. (B) Hybridization with Ig(κ), TCRβ-J1, and TCRβ-J2 of HindIII-digested DNA from spleen (lane 1) and thymic (lane 2) tumors of an SL3-3dm-infected mouse (no. 499-30) diagnosed with histiocyte-associated DLBCL. Arrows mark weak rearranged bands. (C) HindIII-digested DNA from SL3-3dm-induced tumors of the CD11b+ immunophenotype hybridized with probes as indicated. Results are presented for 7 tumors from three different mice diagnosed with myeloid leukemia with maturation. Lanes: 1 and 2, spleen and lymph node tumors of mouse no. 499-10; 3 and 4, spleen and lymph node tumors of mouse no. 499-16; 5 to 7, spleen, cervical, and mesenteric lymph node tumors of mouse no. 498-5. Results are also shown for a negative control (lane C, DNA from the spleen of a noninjected inbred NMRI mouse). (D) DNA from SL3-3dm-induced CD43+ only type tumors cleaved with HindIII and analyzed with Ig(κ), J1, J2, and env hybridization probes or cleaved with EcoRI and hybridized with the IgH probe. Results are displayed for tumors from two mice diagnosed with erythroleukemia (lanes 1 and 2, spleen and mesenteric lymph node tumors of mouse no. 499-24) and myeloid leukemia without maturation (lanes 3 and 4, spleen and thymic tumors of mouse no. 499-25). (E) Hybridization with the ecotropic env gene-specific probe of HindIII-cleaved DNA from two tumors of different types (lane 1, spleen, myeloid leukemia with maturation; lane 2, mesenteric lymph node, DLBCL-HA) found in the same mouse (no. 499-33). Arrows mark the detected bands.