. 2004 Dec;78(23):13216–13231. doi: 10.1128/JVI.78.23.13216-13231.2004

TABLE 2.

Characterization of SL3-3dm-induced tumors by flow cytometry and molecular analysis

Tumor phenotype^a	No. of mice^b	No. of tumors^c	Location of tumor tissue^d			Ecotropic env positive^e	DNA rearrangements in^f:
Tumor phenotype^a	No. of mice^b	No. of tumors^c	S	LN	T	Ecotropic env positive^e	TCRβ	Ig(κ)	1gH
CD3⁺	13	26	4	16	6	17/21	10/21^g	3/21^g	6/21^g
CD3⁺ CD138⁺	1	3	1	1	1	2/2	2/2	0/2	2/2
CD3⁻ CD4⁺	1	2	1	1	0	2/2	2/2	0/2	1/1
CD11b⁺	14	23	14	9	0	17/18	0/18	0/18	0/19
CD43⁺ only	7	12	5	6	1	10/12	0/12	0/12	0/12
CD138⁺ CD43⁺	1	1	1	0	0	1/1	ND^h	ND	0/1
Heterogeneous	1	1	1	0	0	1/1	0/1	0/1	0/1

Immunophenotype determined by three-color flow cytometry with antibodies to CD3, B220, CD11b, CD4, CD8a, IgM, IgD, CD43, and CD138.

Number of mice, some with tumors of more than one type.

Number of analyzed tumors of each immunophenotype.

S, spleen; LN, lymph node (mesenteric or cervical); T, thymus.

Number of tumor samples with clonal ecotropic provirus integration(s)/number of tumor samples analyzed (as detected by Southern blot hybridization).

As determined by Southern blot analysis of genomic tumor DNA using TCRβ (J1 and J2)-, Ig(κ)-, and IgH-specific hybridization probes (number of tumor samples with rearrangement/number of tumor samples analyzed).

See the text for further description of different tumor types in the CD3⁺ subgroup.

ND, not determined.