FIG. 5.

Effects of AMD3451 on the Ca²⁺ flux induced by CXCL12, CCL2, CCL3, CCL4, CCL5, and CCL7 in freshly isolated human monocytes. After loading with the fluorescent calcium indicator Fluo-3, the cells were preincubated for 10 min with (open symbols) or without (closed symbols) compound and then stained with chemokine (50 ng/ml). The transient increase in intracellular calcium concentration was recorded by monitoring the change in fluorescence of the cells as a function of time by using the FLIPR. Each data point represents the average fluorescence of four identical microplate wells. The data shown are from one representative experiment out of three.