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. 2004 Dec;78(23):12868–12876. doi: 10.1128/JVI.78.23.12868-12876.2004

FIG. 2.

FIG. 2.

Northern blot analysis of VA1 expression in transfected or infected 293T cells and its effect on miR-30 production. (A) The level of VA1 expression in 293T cells infected with wild-type (WT) adenovirus at an MOI of 2 or mock infected or transfected with the indicated level of pBS-VA1 was determined by Northern blot analysis. The transfection efficiency was ∼70%. (B) Effect of VA1 on mature and pre-miR-30 RNA expression in 293T cells. 293T cells were cotransfected with pCMV-miR-30 or pSUPER-miR-30, together with pBS-VA1 or the pBS control plasmid, and the pre-miR-30 and mature miR-30 expression levels were determined by Northern blot analysis of nuclear (N) or cytoplasmic (C) RNA fractions. 5S rRNA was used as a loading control.