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Subcellular localization of NS5B insertion sequence mutants. U-2 OS cells were transiently transfected with pCMVNS5Bcon, -R568/570A, -LVL, -R591A, -ΔC12, and -ΔC21, as indicated. At 36 h posttransfection, the cells were subjected to immunofluorescence staining with MAb 5B-12B7 against NS5B. The slides were analyzed by confocal laser scanning microscopy as described in Materials and Methods.
