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Membrane association of NS5B insertion sequence mutants. Hypotonic lysates of U2-OS cells transiently transfected with pCMVNS5Bcon, -R568/570A, -LVL, -R591A, and -ΔC12 were analyzed by membrane flotation as described in Materials and Methods. Low-density (LD) and high-density (HD) fractions were collected, separated by SDS-12% PAGE, and analyzed by immunoblotting with MAb 5B-3B1 against NS5B.
