TABLE 1.
Oligonucleotide sequences
| Oligonucleotide | Sequence (5′-3′)a | Restriction enzyme site(s) |
|---|---|---|
| R568/570A | GTAGATGCCTACCCCTGCAGCGAGCAGGAGTAGGCAAAACCAGAACCAGGCGGGCGCGGCATGAGACACGCTGTG | PstI |
| LVLfwd | CTGGTACTCATCTACCTCCTCCCCAACCGATAAT | PstI, XbaI |
| LVLrev | CTAGATTATCGGTTGGGGAGGAGGTAGATGAGTACCAGTGCA | XbaI, PstI |
| R591Afwd | GGGGTAGGCATCTACCTCCTCCCCAACGCTTAAT | PstI, XbaI |
| R591Arev | CTAGATTAAGCGTTGGGGAGGAGGTAGATGCCTACCCCTGCA | XbaI, PstI |
| S UGA/Xbal | CCAACCGATGATCTAGACGGGGAGCTAAAC | |
| A UGA/Xbal | GTTTAGCTCCCCGTCTAGATCATCGGTTGG | XbaI |
| A1bΔC12 | CGGTCTAGAGTGTTTAGCTCCCCGTTCATCGGTTGGGGAGTAGATAGATGCCTACCCCTACTCAAAGTAGGAG | XbaI |
| S1bR568/570A | CCTGTCTCGTGCCGCACCCGCCTGGTTCATGTG | |
| A1bR568/570A | CACATGAACCAGGCGGGTGCGGCACGAGACAGG | |
| A1bLVL | CGGTCTAGAGTGTTTAGCTCCCCGTTCATCGGTTGGGGAGTAGATAGATGAGTACCAGTACAGAAAGTAG | XbaI |
| A1bR591A | CGGTCTAGAGTGTTTAGCTCCCCGTTCATGCGTTGGGGAGTAG | XbaI |
a
Mutagenic nucleotides are double underlined. Restriction enzyme recognition sites are underlined, and stop codons are shown in bold. The Pstl sites in LVLfwd and LVLrev were preserved only in the overhanging nucleotides due to the introduced mutations.