FIG. 1.
Development of neurological disease in wild-type and TNF-α−/− mice following infection with Fr98 (A) or FrCasE (B). Neonatal wild-type and TNF-α−/− mice were inoculated with 104 FFU of virus by i.p. injection, prior to 24 h postbirth. Mice were then followed for the development of clinical signs of severe ataxia and seizures (A) or paralysis (B). Data are presented as the percentage of mice with severe neurological disease for 22 wild-type and 16 TNF-α−/− mice for panel A and 7 wild-type and 6 TNF-α−/− mice for panel B. Statistical analysis was done by Kaplan-Meier survival curve analysis with GraphPad Prism (GraphPad, San Diego, Calif.). The P value for the two-tailed log rank test for trend between Fr98 virus-infected wild-type and TNF-α−/− mice was <0.0001.