Intracellular localization of transiently expressed gK. Cells transfected with pgKDIV5 were fixed at 25 h posttransfection and stained with anti-V5 antibodies for gK (red) (A, B, and C) or specific organelle markers (green) that recognized the ER (A), Golgi (B), or plasma membranes (C). Magnification, ×63; zoom, ×4.