Intracellular localization of UL20p in transfected (A to C), ΔgK/UL20amFLAG (D and E), or gKDIV5/UL20amFLAG (F) virus-infected cells. Cells were fixed at either 24 h posttransfection (A to C) or 12 hpi (D to F) and stained with anti-FLAG antibodies for UL20p (red) or specific organelle markers (green) that specifically recognized ER (A and D), Golgi (B, E, and F), or plasma membranes (C). Magnification, ×63; zoom, ×4 (A to C) or ×2 (D to F).