FIG.7.

Colocalization of gK and UL20p in transient-coexpression experiments with or without UL20p containing an ER retention motif. Vero cells coexpressing gK (green) and either UL20amFLAG (A), the ER retention control motif protein UL20p(KKSLAL) (B), or the ER retention motif protein UL20p(KKSL) (C) were fixed at 24 h posttransfection, and the subcellular distributions of gK (green) and UL20p (red) were determined. Fluorograms, which depict pixel intensity and distribution, are shown for each image subset, as well as the fluorogram regions that showed significant colocalization between gK and UL20p (panel 2 in A, B, and C). Image correlation masks where significant specific colocalization occurred are shown for each image subset (panel 3 in A to C). In addition, a negative image correlation mask is shown with a blue-masked image depicting the specific subcellular region of significant colocalization (panel 4 in A to C). Magnification, ×63; zoom, ×4.