FIG. 1.

Depletion of CD4⁺ CD25⁺ T cells in C57BL/6 mice. Mice were injected intraperitoneally with 1.2 mg of anti-CD25 monoclonal antibody clone PC61. Four or 5 days later, peripheral blood mononuclear cells were stained with CD25⁺-peridinin chlorophyll protein Cy5.5 clone 7D4 and CD4⁺-phycoerythrin. Flow cytometry analysis was done in FACScan (Becton Dickinson), and data were analyzed with CellQuest software.