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. 2004 Dec;78(23):13082–13089. doi: 10.1128/JVI.78.23.13082-13089.2004

FIG. 2.

FIG. 2.

CD8+ T-cell primary response to vaccination after depletion of CD4+ CD25+ T cells. C57BL/6 mice were either depleted of CD25+ T cells or injected with isotype immunoglobulin. Five days later, the animals were vaccinated with gB-DNA intramuscularly or SS-CpG in the footpad. Control mice were injected with either β-galactosidase DNA or nonbioactive CpG1982 or -2138. HSV infection of depleted and nondepleted mice was used as a positive control. Spleen and draining lymph nodes were collected for analysis on either day 7 postimmunization for SS-CpG or day 12 for gB-DNA vaccination. IFN-γ ELISPOT and intracellular staining were performed as described in Materials and Methods. (A) IFN-γ ELISPOT for gB-DNA vaccination and HSV infection, (B) ELISPOT for SS-CpG vaccination, (C) intracellular staining for IFN-γ gB-DNA- and SS-CpG-vaccinated mice. The percentage shown in each cytogram represents the mean of IFN-γ-producing CD8+ T cells obtained from each of four mice per group in three separate experiments. *, statistically significant (P ≤ 0.05) compared to isotype immunoglobulin- and CpG1982-treated groups.