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. 2004 Dec;78(23):13082–13089. doi: 10.1128/JVI.78.23.13082-13089.2004

FIG. 3.

FIG. 3.

CD8+ T-cell memory response to vaccination following depletion of CD4+ CD25+ T cells. C57BL/6 mice were either depleted of CD25+ T cells or injected with isotype immunoglobulin. Five days later the animals were immunized with gB-DNA intramuscularly or SS-CpG in the footpad. Control mice were injected with either β-galactosidase DNA or nonbioactive CpG 1982 or 2138. HSV infection of depleted and nondepleted mice was used as a positive control. Spleen and draining lymph nodes were collected for analysis on day 60 postimmunization. IFN-γ ELISPOT and intracellular staining were performed as described in Materials and Methods. (A) ELISPOT for gB-DNA vaccination and HSV infection, (B) ELISPOT for SS-CpG vaccination, (C) intracellular staining for gB-DNA- and SS-CpG-vaccinated mice. The percentage shown in each cytogram represents the mean of IFN-γ-producing CD8+ T cells obtained from each of four mice per group in two experiments performed. *, statistically significant (P ≤ 0.05) compared to isotype immunoglobulin- and CpG1982-treated groups.