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. 2004 Dec;78(23):13207–13215. doi: 10.1128/JVI.78.23.13207-13215.2004

FIG. 1.

FIG. 1.

(A) Schematic representation of the Ad11 genome organization. Shown are the locations of the ITRs, the packaging signal (Ψ), the region encoding proteins required early (E) during infection, and the regions encoding proteins required late (L) during infection. Also shown are the hexon (L2) with the location of the hypervariable region (HVR) and the amino acid homology with Ad35. Likewise, for the adenovirus fiber molecule (L5), position and amino acid homology with Ad35 are shown for fiber tail, fiber shaft, and fiber knob regions. (B) Schematic representation of the Ad11 plasmid system generated to allow easy generation of replication-deficient recombinant Ad11 vector. The pAdApt11 plasmid contains nt 1 to 4669 of the Ad11 genome with an expression cassette instead of E1 sequences. Cosmid pWE.Ad11 contains nt 3401 to 34794 of the Ad11 genome. The two DNAs both have an identical 1,268-bp fragment that mediates homologous recombination in PER.C6/55K packaging cells. (C) Typical banding pattern obtained after CsCl gradient separation. The arrow denotes the position in the gradient of mature Ad5 and Ad11 virions.