TABLE 1.

Tropism of Ad11 and Ad35 recombinant vectors^a

Cell line	Tissue type	% CD46 expression	% eGFP+ cells		Median fluorescence
			Ad11	Ad35	Ad11	Ad35
K562	Erythroid	100	78.6	75.3	23.9	22.2
SK-N-MC	Neuronal	0	43.2	51.4	23.3	26.7
MCF-7	Breast	98	99.7	98.0	4,043.0	5,953.0
HS766T	Pancreas	100	99.8	99.8	9,589.0	9,493.0

^aCells were seeded at 10⁵ cells per ml in 6-well plates and subsequently exposed to 1,000 vp (per cell) of either Ad11 or Ad35 vector carrying eGFP. Forty-eight hours later, cells were harvested, washed, and subjected to FACS analysis. Background fluorescence of each cell population was set at 1% with nontransduced cells. Both the percentage of GFP-positive (GFP⁺) cells as well as the median fluorescence per cell are shown. Cell membrane expression of the BC-1 isoform of CD46 was determined by using antibody CD46 (WS IV) clone 122.2 at a 1-in-50 dilution (stock concentration, 2.57 mg/ml). As a negative control, secondary antibody fluorescein isothiocyanate- labeled goat anti-mouse immunoglobulin alone was tested at the same concentration.