FIG. 1.

PRV infection induces COX-2 mRNA and protein expression. (A) Real-time PCRs were performed on total RNAs from PRV-infected REF cells (MOI = 5) at 8 hpi with COX-1- or COX-2-specific primers. The data were compared to previously published microarray data (39) for COX-1 and COX-2 mRNA levels. (B) RIPA lysates from PRV-infected REF cells (MOI of 5 PFU/cell) were collected at the indicated times postinfection and analyzed by Western blotting with rabbit polyclonal antibodies against COX-1 and COX-2 (top two panels). The membrane was probed with an anti-actin antibody to provide a loading control (bottom panel).