FIG. 2.

COX inhibitors cause a dramatic reduction in PRV infectious yield. REF cells were infected with PRV (MOI = 1) in the presence of increasing concentrations of indomethacin (A), NS398 (C), or FR122047 (E). At 24 hpi, the cells and supernatants were collected, freeze-thawed three times, and titrated on Vero cells. For determinations of the cytotoxicities of the COX inhibitors, uninfected REF cells grown in 96-well dishes were treated with increasing concentrations of indomethacin (B), NS398 (D), or FR122047 (F) for 24 h. Cell Titer Aqueous One solution was used to determine the percentages of metabolically active cells relative to cells that were not treated with a drug. Error bars represent the standard deviations of at least three experiments.