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PRV DNA replication is reduced in the presence of indomethacin. REF cells were infected with PRV (MOI = 5) in the presence of 0.25% DMSO or 500 μM indomethacin. At the indicated times postinfection, samples were collected for total DNA isolation and were analyzed by slot blotting with a random-primed DIG-labeled probe for the entire PRV genome. A mock-infected sample was included to assess the level of nonspecific hybridization to contaminating host DNA.
