FIG. 7.

COX inhibitors cause an accumulation of empty capsids in the nuclei of PRV-infected cells. REF cells were infected with PRV Becker at an MOI of 5 PFU/cell in the presence of no drug (A and B) or 500 μM indomethacin (C and D). At 12 h postinfection, the cells were fixed and processed for transmission electron microscopy. The numbers of full capsids in 25 to 30 images were counted for each treatment to determine the median number of full capsids per frame. Representative images showing the median numbers of full capsids in control or indomethacin-treated cells are shown (A and C). In addition, the frames with the maximum numbers of full capsids per frame for each treatment are shown (B and D) to provide an idea of the range of observations.