FIG. 4.

RK-BARF0 binds scramblase but does not induce proteasome-dependent degradation. (A) Flag- and His6-tagged RK-BARF0, RK-179, and RK-158 were transiently transfected into H1299 cells with myc-tagged scramblase. Lysates were directly loaded onto an SDS-PAGE gel (left panel) or were immunoprecipitated (IP) with α-Flag (right panel). RK-BARF0 was detected with α-His6, and scramblase was detected with α-cMyc. (B) RK-BARF0 and RK-158 were coexpressed with scramblase and were treated with 20 μM MG132 to examine degradation of scramblase. Scramblase was detected with α-cMyc.