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. 2004 Dec;78(23):12848–12856. doi: 10.1128/JVI.78.23.12848-12856.2004

FIG. 6.

FIG. 6.

Purification of RK-BARF0 specific antisera. (A) The sequence of the peptides used to inoculate rabbits are underlined within the RK-BARF0 amino acid sequence. The EBV coordinates corresponding to the B95-8 genome are indicated along with RK-BARF0 and BARF0 start sites, the RK-BARF0 splice site, its corresponding exons (Va′ and VIIb), and amino acid 158. (B) Lysates from DH5α expressing GST-BARF0 and GST RK-158 were probed with 2157 and 2161 sera to confirm reactivity. (C) Serum 2157 was purified against immobilized GST-BARF0, and serum 2161 was purified against immobilized GST-RK-158. Purified sera and column flowthrough (Flow Thru) were tested against DG75, COS-1, and COS-1 RK-BARF0 cell extracts.