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RK-BARF0 is not detected in EBV-positive cell lines and in tumors. The purified serum 2157 was used to screen multiple EBV-positive and -negative cell lines and tumors. COS-1 cells expressing His6-tagged RK-BARF0 were used as a positive control. The serum was used to screen whole-cell lysates (A and B) and fractionated cell lysates (C and D) as well as MG132-treated cells (D). C-term, C terminal; WB, Western blot.
