FIG. 5.

Virus cell binding assay. Macrophage single-cell suspensions were obtained from primary cultures, incubated for 1 h at 4°C with 100 PFU of virus/cell, washed extensively with ice-cold PBS, and fixed with 4% PFA. Fixed cells were reacted with an anti-TMEV polyclonal serum followed by a FITC-conjugated goat anti-rabbit immunoglobulin. Labeled cells were analyzed using a FACSCalibur flow cytometer and the CellQuest software. The abscissa is fluorescence intensity, and the ordinate is the number of events.