TABLE 1.
Oligonucleotide primers used for PCR
| Oligonucleotide | Sequence (5′→3′)a | Annealing site (5′ end)b |
|---|---|---|
| gEΔC up | TGATCACCGAACCGGGGCAAC | 117677 |
| gEΔC rev | TGAAGCCGTACAGATTAAAAATA | 117490 |
| ←DVGAAKV | ||
| AYRVup | GTCTACCCCCGCGGCTTTAAC | 117523 |
| SstII | ||
| AYRVrev | AAGTCCCCGTATAACCAAAGC | 117524 |
| ←LGAGYM | ||
| YAGLup | GGAAGGCCGGCGCCATACATG | 117564 |
| NarI | ||
| YAGLrev | AGTGGACGATTTCGAGGACTC | 117565 |
| DAEAADAEE→ | ||
| SSTTlo | AGGACGCGGAAGCAGCTGATGCGGAAGAAG | 117608 |
| PvuII | ||
| SSTTrev | AGTTTGGTAACGCGATTGGAG | 117609 |
| AvrII-ORF68 | CACATTCCCTAGGAAACCCGTTTG | 115529 |
| AvrII | ||
| ORF68-AvrII | GCATCCGCGCCTAGGTATATGACC | 117899 |
| AvrII | ||
| pgEf | AACTAATTATCCCGGATTTT | 115561 |
| pgEb | TCACCGGGTCTTATCTATAT | 117679 |
a
Altered bases and amino acids are boldfaced; arrows indicate direction of protein synthesis.
b
VOka sequence, GenBank accession number NC_001348.