FIG. 4.

The WW domain but not the PDZ-binding motif in TAZ is essential for binding to polyomavirus T antigens. Wild-type GST-TAZ (TAZ), GST-TAZΔPDZ (lacking residues 386 to 395; TAZ ΔPDZ), and GST-TAZΔWW (lacking residues 125 to 156; TAZ ΔWW) constructs were transfected into NIH 3T3 cells. Transfected cells were then infected by wild-type virus. Cell extracts were prepared, and GST pull-down assays were performed 24 h postinfection. After SDS-PAGE, Western blot analyses for T antigens and TAZ were performed. A total of 5% of cell lysates were loaded in input lanes.