FIG. 2.
Cell cycle-dependent histone modifications and MCM protein binding at the KSHV TR. (A) Fluorescence-activated cell sorter analysis of propidium iodide-treated BCBL1 cells grown as asynchronous cultures or arrested in late G1 with mimosine or in G2/M phase with colchicine. (B) Real-time PCR analysis of ChIP assays with antibodies to AcH4, AcH3, MeH3K4, LANA, ORC2, MCM3, or IgG at KSHV loci A, G, and K as depicted in Fig. 1A. Asynchronous (black), mimosine-arrested (grey), and colchicine-arrested (hatched) cultures are indicated in the bar graph. (C) Western blot analysis of total cellular proteins derived from asynchronous (ASYN), mimosine-arrested (G1), or colchicine-arrested (M) cells probed with antibodies to LANA or β-actin, as indicated.