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. 2004 Nov;78(22):12566–12575. doi: 10.1128/JVI.78.22.12566-12575.2004

FIG. 3.

FIG. 3.

Nucleosome organization at TR. (A) Southern blots of MNase I-digested BCBL1 nuclei. Nuclei were digested with a twofold serial increase in MNase I, ranging from 15 to 120 U/100 μl of reaction mixture as indicated above. Extracted DNA was probed with sequences specific for the LBS, TR, or ORF50 region as indicated above each blot. The arrow indicates mononucleosome fragments of ∼147 bp. (B) MNase I digestions of asynchronous (A), mimosine-arrested (G1), or colchicine-arrested (M) BCBL1 cells were probed with sequences specific for the TR. MNase I-digested samples were cut with NotI (even-numbered lanes) or not restricted (odd-numbered lanes) prior to electrophoresis.