FIG. 5.

LANA recruits ORC to TR. 293 cells transfected with 2 × TR plasmid and either LANA expression vector (black bars) or empty vector (grey bars) were then assayed by ChIP assay and real-time PCR. PCR primers specific for the LBS region of TR or the AMP gene of the plasmid were analyzed by ChIP assay with antibodies specific for LANA, ORC2, MCM3, AcH3, and IgG. Data from at least three independent ChIP experiments were normalized as the percentage of total input DNA recovered for each antibody.