FIG. 1.
FACS detection of RV-reactive B lymphocytes. Examples of adult, infant, and neonate blood samples are presented. The cells were first gated on CD19pos cells, and dot plots of GFP-VLP- versus DsRed-VLP-reactive B cells were then created. In each panel, results of analyzing approximately 5 × 105 CD19pos B cells are shown. Gates of fluorescence positivity were determined by comparison with identical B cells incubated without VLP (top panels).