FIG. 5.

FACS detection of CD27^neg naive and CD27^pos memory B cells reacting with VLP2/6 and VLP2/6/7. An example of dot plotting with CD27-PE- versus CD19-APC-labeled cells is presented (A). Dot plots of GFP-VLP2/6 (B) and GFP-VLP2/6/7 (C) versus CD27-PE-labeled cells gated on CD19^pos cells were created. The number of CD19^pos B cells analyzed were 5 × 10⁵ (B) and 10⁶ (C), respectively. Gates of fluorescence positivity were determined by comparison with identical B cells incubated without VLP (B and C, top panels).