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. 2004 Nov;78(22):12557–12565. doi: 10.1128/JVI.78.22.12557-12565.2004

FIG. 5.

FIG. 5.

Expression and interaction of M and N gene products in vitro and model of the interactions leading to the formation of SARS-CoV. (A) Schematic representation of M expression vector and mutants. The ability of the indicated mutants to form nucleocapsids in combination with N is shown. The diagram shows the assembly of VLPs by cotransfection of M mutants with S and N. (B) Immunoprecipitation and coassociation of in vitro translated M and indicated mutants by using recombinant His-tagged N protein. Proteins were transcribed and translated in vitro with reticulocyte lysate and immunoprecipitated by a polyclonal anti-His tag antiserum and protein G agarose. (C) Transfections and transmission EM analysis were performed as described in the legend of Fig. 1 (magnification, ×20,000). (D) Coassociation of in vitro translated M and E, but not N or a mutant HIV envelope protein gp145 (2), with S. Proteins were transcribed and translated in vitro with reticulocyte lysate (left) and immunoprecipitated by a human monoclonal antibody to SARS S (middle) or to HIV Env (right) and protein G agarose as described in Materials and Methods.