Table 2. Proteomic Analysis: Protein Expression that Increased in GSK-3βI, HCI and LCI Groups Compared to HCC Group.
Proteins in pink were increased in the GSK-3βI and LCI group compared to the control. Proteins in yellow were increased in the GSK-3βI and HCI group compared to the control. Proteins in green were increased in the HCI and LCI group compared to the control. Proteins listed in blue were increased in all three experimental groups compared to the control. All proteins listed showed statistically significant differences in expression between appropriate experimental group (GSK-3βI, HCI and LCI) and the HCC group by Scaffold or ProteoIQ analysis. Pink indicates proteins that were increased in GSK-3βI and LCI group compared to the control. Orange indicates proteins that were increased in the GSK-3βI and HCI group compared to the control. Green indicated proteins that were increased in the HCI and LCI group compared to the control. Blue indicates proteins that were increased in all three experimental groups (GSK-3βI, LCI and HCI) compared to the control. HCC High Cholesterol Control, LCI- Low Dose Calpain Inhibitor, HCI- High Dose Calpain Inhibitor, GSK-3βI- GSK-3β Inhibitor
| GSK-3p Protein Expression> HCC | LCI Protein Expression>HCC | HCI Protein Expression>HCC |
|---|---|---|
| 60S ribosomal protein L32 | 26S proteasome non-ATPase regulatory subunit 5 | Alpha-soluble NDK attachment protein |
| AHNAK nucleoprotein | 60S ribosomal protein L32 | Annexin A6 |
| Alpha-actinin-1 | Apolipoprotein A-1 | Armadillo repeat containing protein 10 |
| Alpha-actinin-4 | Calpain-1 catalytic subunit | Beta-2-syntrophin |
| Alpha-soluble NSF attachment protein | Glucosamine-6-phosphate isomerase | Calpain-1 catalytic subunit |
| Annexin A6 | Homer protein homolog 1 (Homer-1) | Calreticulin |
| Apolipoprotein E | Integrin-linked protein kinase | Coiled-coil domain-containing protein 141 |
| Beta-catenin | MHC class II antigen | Fatty-acid amide hydrolase 1 |
| Calreticulin | Mitochondrial import inner membrane translocase subunit TIM50 | Fumarate hydrolase, mitochondrial |
| Cat eye syndrome critical region protein | MYL2 | Glucosamine-6-phosphate isomerase |
| Chaperorin containing, TCP | Myomesin-3 (Myomesin family member 3) | GTP-AMP phosphotransferase AK3 |
| Complexin-1 | Myosin-9 | GTP-binding nuclear protein Ran |
| Cysteine-rich motor neuron 1 protein | Myotilin | Homer IF (Homer protein homolog 1) |
| Cytoplasmic aconitate hydratase | NADH-ubiquinone oxidoreductase chain 4 | Myomesin-3 |
| Domain-containing protein 4 | Polypyrimidine tract binding protein 1 | Myotilin |
| Integrin alpha-V | Probably C->U editing enzyme APOBEC-2 (EC3.4.5) | NADH-ubiquinone oxidoreductase chain 4 |
| LIM domain only protein 7 | Protein CDV3 homolog | NAP1L1, NRP |
| Myomesin-3 | Pyridoxal kinase (EC 2.7.1.35) Pyridoxine kinase | Phosphoacetylglucosamine mutase |
| Myotilin | Ribosomal Protein | Proteasome subunit alpha type 6 |
| NAD(P) transhydrogenase, mitochondrial | Ryanodine receptor 2 | Protein NipSnap homolog 3A |
| NADH dehydrogenase (ubiquinone)1 | Tropomodulin-1 (Erythrocyte tropomodulin) | RuvB-like 1 |
| Obscurin | Voltage-dependent anion- selective channel protein 2 (VDAC-2) | Sorting nexin 18 |
| Peptidyl-Prolylcis-trans isomerase | Spectrin alpha chain, non-erythrocytic 1 | |
| Protein CDV3 homolog | Synemin (Desmuslin) | |
| Pyridoxal kinase | Talin-1 | |
| RuvB like 1 | Uncharacterized Protein TMEM70 | |
| Synemin | ||
| Talin-1 | ||
| Tubulin beta chain | ||
| Uncharacterized protein (ILK) | ||
| Uncharacterized protein (FN1) | ||
| Unconventional myosin-X | ||
| Vitamin D binding protein |