Table 3. Proteomic Analysis: Expression of Proteins that was Decreased in GSK-3βI, HCI and LCI Groups Compared to HCC Group.
Proteins in pink were decreased in the GSK-3βI and LCI group compared to the control. Proteins in yellow were decreased in the GSK-3βI and HCI group compared to the control. Proteins in green were decreased in the HCI and LCI group compared to the control. Proteins listed in blue were decreased in all three experimental groups compared to the control. All proteins listed showed statistically significant differences in expression between appropriate experimental group (GSK-3βI, HCI and LCI) and the HCC group by Scaffold or ProteoIQ analysis. Pink indicates proteins that were decreased in GSK-3βI and LCI group compared to the control. Orange indicates proteins that were decreased in the GSK-3βI and HCI group compared to the control. Green indicated proteins that were decreased in the HCI and LCI group compared to the control. Blue indicates proteins that were decreased in all three experimental groups (GSK-3βI, LCI and HCI) compared to the control. HCC- High Cholesterol Control, LCI- Low Dose Calpain Inhibitor, HCI-High Dose Calpain Inhibitor, GSK-3βI- GSK-3β Inhibitor
| GSK-3P Protein Expression< HCC | LCI Protein Expression<HCC | HCI Protein Expression<HCC |
|---|---|---|
| Alpha-1,4, glucan phosphorylase | Alanine aminotransferase 1 (ALT1) | 14-3-3 protein theta |
| Fibrinogen beta chain | Alpha-1,4 glucan phosphorylase | 40S ribosomal protein SA |
| Ribonuclease inhibitor | ATP synthase F(0) complex subunit B1, mitochondrial | Alanine-glyoxylate aminotransferase |
| Serum deprivation-response protein | ATP Synthase protein 8 | Antithrombin protein |
| Succinyl-CoA ligase subunit beta | Carbonic Anhydrase 1 | ATP synthase protein 8 |
| Uncharacterized Protein (CHCHD3) | Cytochrome c oxidase subunit 5A | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
| Uncharacterized Protein (COX6B) | Fibrinogen beta chain | Cytochrome c oxidase subunit 5A |
| Uncharacterized Protein (PURA) | Hemoglobin subunit alpha (Alpha globin) ( Hemoglobin alpha chain) | Glyceraldehyde-3-phosphate dehydrogenase |
| Uncharacterized Protein (COX5A) | LETM1 and EF-hand domain containing protein 1, mitochondrial | Glycogen phosphorylase |
| Voltage-dependent anion selective channel protein 1 (VDAC 1) | Hemoglobin subunit alpha | |
| V type proton ATPase catalytic subunit A | LETM1 and EF-hand domain containing protein 1, mitochondrial | |
| Malate dehydrogenase, mitochondrial | ||
| NADH ubiquinone oxidoreductase chain 4 | ||
| Peptidyl-prolyl cis-trans isomerase | ||
| Succinate dehydrogenase | ||
| Succinyl-CoA ligase subunit beta | ||
| Voltage dependent anion-selective channel protein 1 (VDAC-1) |