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. Author manuscript; available in PMC: 2018 Jan 20.
Published in final edited form as: ACS Synth Biol. 2016 Aug 9;6(1):39–44. doi: 10.1021/acssynbio.6b00160

Figure 4.

Figure 4

Detection of d-Phe-l-Pro DKP by LC–MS/MS and comparison to synthetically prepared DKP. (A) Retention time comparison of d–l, l–l, and CFPS-produced DKP to determine the stereochemistry of the DKP produced by CFPS. This panel also shows that SFP is required for DKP production. (B) Time-dependent increase in m/z 245.128 signal after Sfp is added to the CFPS reaction. Data points are the average of two technical replicates. (C) Comparison of the fragmentation pattern of the CFPS-produced (top) and synthetically prepared DKP (bottom). The spectrum at the bottom of panel C is annotated with predicted fragment ion structures.