FIG. 2.

Prototype and mutant proteins used to characterize the HCV RdRp activities and an initial survey of the effects of the mutations. The activities of Δ21 and mutant proteins affected in the residues identified by Bressanelli et al. (3) to bind NTP. Names of the proteins used in the reactions are underlined at the top of the autoradiograms. The presence or absence of Mn²⁺ at 1 mM and GTP at 200 μM are indicated by plus and minus signs, respectively. The size of each band (in nucleotides) is noted to the left of the autoradiograms.