FIG. 5.

Production of template switch products by mutant D225. (A) Autoradiogram of the products made by Δ21 and mutant R222A/D225A and D225Y. The relevant conditions for the reactions are indicated above the autoradiogram. (B) Quantification of the oligomeric products made by Δ21 and D225A using three concentrations of template 14-2 (12.5, 62.5, and 125 nM represented as 1-, 5-, and 10-, respectively), which was previously characterized for template switch (16). The amounts of the products made were normalized for the amount of radiolabeled CMP incorporated and plotted as ratio of monomer to template switch products.