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Effects of mutations on the recognition of the template initiation nucleotide
| Protein | With Mn2+
|
Without Mn2+
|
||
|---|---|---|---|---|
| +1C | +1U | +1C | +1U | |
| Δ21 | 100 | 11 | 100 | 12 |
| R158A | 12 | 1 | 0 | 0 |
| S367A | 88 | 8 | 50 | 15 |
| R386A | 75 | 8 | 0 | 0 |
| R394A | 53 | 5 | 0 | 0 |
| R48A | 59 | 1 | 0 | 0 |
| D225A | 205 | 1 | 0 | 0 |
| R158A/R386A | 0 | 0 | 0 | 0 |
| R158A/R394A | 0 | 0 | 0 | 0 |
| R158A/S367A/R386A | 0 | 0 | 0 | 0 |
