TABLE 3.

Summary of the activities of the mutations tested with RNA LE19 in this study^a

Protein	Without Mn2+				With Mn2+
	Primer extension	Template switch	De novo initiation	TNTase	Primer extension	Template switch	De novo initiation	TNTase
Δ21	++	++	++	++	++	++	++	++
S367A	++	+	+	+	++	++	++	++
R158A	−	−	−	−	++	++	+	+
R386A	+	−	−	−	++	++	+	++
R394A	+	−	−	−	++	+	+	+
R48A	+	−	+	+	++	+	+	++
D225A	+	−	−	+	++	−	++	++

^a++, high level of activity; +, detectable; −, not detected.