Figure 5. Dusp15 represses myelin gene expression.
Mag, Mbp, Cx32, Pmp22 and Srebp-1c mRNA levels were measured by quantitative RT-PCR in (A) RT4 Schwann cells, Dusp15 and Mag in (B) Primary Schwann cells treated with siRNA against Dusp15 and in (C and D) Dusp15-KO Schwann cell line compared to parent RT4 Schwann cells. (E) Mbp and (F) Pmp22 mRNA levels were measured in Dusp15-KO Schwann cells treated with ectopic expression levels of Dusp15. All experiments represent biological triplicates, and expression levels were normalized to 18S rRNA. (*P<0.05)