Table 2.

Primary Abs used in this study^*

Antigens	Clones & Producers	Fixation	Antigen Retrieval Conditions
CD4**	1F6 Vector (VP-C318)	PFA	1mM EDTA buffer pH8 98°C 20min
CD8α	1A5 Vector (VP-C325)	PFA	10mM citrate buffer pH6 98°C 20min
CD68	KP1 DAKO M0814	PFA	10mM citrate buffer pH6 98°C 20min
CD163	Vector VP-C374	PFA	10mM citrate buffer pH6 98°C 20min
BDCA-2	104C12.08 Dendritics DDX0041	SAFEFIX II	1mM EDTA buffer pH8 98°C 20min
CD123	V-18 Santa Cruz sc-681	PFA	10mM citrate buffer pH6 98°C 20min
c-Kit	Novus Biologicals NBP1- 85593	SAFEFIX II	10mM citrate buffer pH6 98°C 20min
CCL3	Neomarkers RB-10489-P	PFA	10mM citrate buffer pH6 98°C 20min
CCL4	R&D AF-271-NA	PFA	10mM citrate buffer pH6 98°C 20min
CCL5	R&D AF-278-NA	PFA	10mM citrate buffer pH6 98°C 20min
CCL20	2069D.05 / Dendritics & # DDX0420	PFA	1mM EDTA buffer pH8 98°C 20min
IL-8	Abcam ab7747-500	PFA	10mM citrate buffer pH6 98°C 20min
IP10	R&D AF-266-NA	PFA	10mM citrate buffer pH6 98°C 20min
CCR5	AIDS Reagents 4914	PFA	1mM EDTA buffer pH8 98°C 20min
CCR6	R&D MAB195	PFA	10mM citrate buffer pH6 98°C 20min
CXCR1	Novus Biologicals NBP1- 88143	SAFEFIX II	1mM EDTA buffer pH8 98°C 20min
CXCR2	Novus Biologicals NBP1- 02412	PFA	10mM citrate buffer pH6 98°C 20min
CXCR3	R&D 49801 MAB160	PFA	10mM citrate buffer pH6 98°C 20min
BMK13	Abcam 77842	PFA	10mM citrate buffer pH6 98°C 20min
CD138	AnaSpec 53317	PFA	10mM citrate buffer pH6 98°C 20min
DC- SIGN	R&D MAB161	PFA	10mM citrate buffer pH6 98°C 20min
S100B	Thermo Scientific MA5- 15359	PFA	10mM citrate buffer pH6 98°C 20min
Fascin	Vector Lab VP-F703	PFA	10mM citrate buffer pH6 98°C 20min
CD83	Vector Lab VP-C368	PFA	10mM citrate buffer pH6 98°C 20min
CD1a	DAKO 3571	PFA	10mM citrate buffer pH6 98°C 20min
Langerin	Vector Lab VP-L552	PFA	10mM citrate buffer pH6 98°C 20min
Elastase	DAKO 0752	PFA	10mM citrate buffer pH6 98°C 20min
NKG2A	Epitomics T3308	PFA	10mM citrate buffer pH6 98°C 20min
Tryptase	DAKO M7052	PFA	10mM citrate buffer pH6 98°C 20min

Notes:

^*Isotype controls were performed on all examined animals (See Supplementary Fig. 1).

^**CD4 Ab concenration was optimized to only stain CD4+ T cells that have higher CD4 expression than macrophages³⁶ (See Supplementary Fig. 2).