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. Author manuscript; available in PMC: 2017 Mar 6.
Published in final edited form as: Mucosal Immunol. 2016 Jul 20;10(2):341–351. doi: 10.1038/mi.2016.61

Figure 7. Beta 1 integrin blockade in WT fibrocytes caused decreased Collagen 1 expression with periostin treatment but showed no effect in fibroblasts.

Figure 7

Mesenchymal cells were cultured in complete media, sorted into fibrocytes and fibroblasts, then switched to SFM where they were treated with purified rat anti-mouse beta 1 (β1)(HMβ1-1, rat CD29) blocking antibody or purified armenian Hamster IgG (400916) isotype control for 30mins, then incubated with or without periostin (500ng/mL) for 48hours. Total RNA was isolated and by real time RT-PCR we measured the expression of collagen 1 and β-actin in fibrocytes (A) and fibroblasts (B). Data are representative of mean ±SEM, n=3 wells/treatment per group *p<0.05. and ns=not significant.