FIG. 7.

Requirement of ERK activation for RANTES expression. After mock or JEV (NT113) infection, mixed glia were cultivated in medium alone or in the presence of U0126 (10 μM) or NAC (5 mM). The level of RANTES in the supernatants (24 h postinfection) was determined by enzyme-linked immunosorbent assay (n = 4) (A). The levels of phosphorylated ERK (8 h postinfection), total ERK (8 h postinfection), and JEV NS3 protein (24 h postinfection) were determined by Western blot analysis. One of three separate experiments is shown (B).