FIG. 8.

Activation of RANTES promoter activity in JEV-infected cells. C6 glioma cells were transiently cotransfected with the three RANTES promoter reporters (pGL-A, pGL-B, and pGL-C) and the Renilla luciferase reporter gene. Twenty-four hours later, cells were mock infected or infected with JEV (NT113) for another 36 h. The cells were then lysed, and the lysates were harvested. Firefly and Renilla luciferase activities were detected by the dual-luciferase reporter assay system. Firefly luciferase activity was normalized to Renilla luciferase activity. The mock-infected pGL-A group was the control (n = 3).