Fig. 5.

ARHGDIA knockdown (a) or overexpression (c) affects the GTPase activity of Rho family proteins. Rac1, Cdc42, and RhoA with GTP form represents the GTPase activity, which was analyzed from cell lysis after enrichment by pull-down. Total target proteins (Rac1, Cdc42, and RhoA) were present in whole cell lysate. The integrated density measurements were calculated based on the fold change of active-form protein versus total target protein. The gray value of control treatment (NC) was set as 1. The immunoblot quantitation of GTPase activity was shown in (b) and (d). NC nontargeting control siRNA or pHA empty vector. *p < 0.05, **p < 0.01