Fig. 5.

Single and combined microautoradiography (MAR) and FISH digital images of mechanically homogenized biomass from R1, after anoxic incubation with [³H]acetate (a–d) and [³H]glucose (e–h). (a,e) Microautoradiographic image with black silver grains revealing cells that were active in the incorporation of radioactive labeled substrate. (b,f) Fluorescence in situ hybridization of the microscopic field in panels (a) and (e) with Cy3-labeled Amx820+Bfu613 oligonucleotide probes targeting for anammox populations (AMX, red color allocation). (c,g) Fluorescence in situ hybridization of the microscopic field in panels (a) and (e), respectively, with the FLUOS-labeled EUB-mix oligonucleotide probes (green). (d,h) Overlays of microautoradiographic and FISH digital images, namely (a+b) and (e + f). White arrows indicate the localization of cells that actively incorporated the radiolabeled substrates. White circles indicate the localization of probe-defined anammox cells (scale bars: 20 μm). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)