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. 2017 Jan 1;7(1):41–52.

Figure 2.

Figure 2

Inhibition of PDE-5 suppresses colonic inflammation-induced tumorigenesis. (A) Schema of AOM/DSS treatment and Sildenafil injection. (B) ELISA analysis of PDE-5 expression in colonic tissues from Sildenafil and normal saline (NS) treatment mice (n=5). (C) Representative macroscopic findings of colon tumors induced by AOM/DSS in Sildenafil and normal saline (NS) treatment mice. (D, E) Number of macroscopic tumors (D) and tumor burden of AOM/DSS-treated mice (E) per colon (n=6). (F) Colon length of AOM/DSS-treated mice per colon (n=6). (G, H) H&E staining of colonic tissue after AOM/DSS treatment (G). Scale bar=200 μm. Tumor area (%, tumor area/total area) was calculated (H, n=3). (I, J) PCNA staining of colonic tissue after AOM/DSS treatment (I). Scale bar=50 μm. PCNA positive cell (%, positive cell/total cell) was calculated (J, n=3). (K, L) TUNEL analysis of colonic tissue after AOM/DSS treatment (K). Scale bar=100 μm. Apoptotic cell per frame was calculated (L, n=3).