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Sildenafil inhibits MDSC invasion in vitro. (A) ELISA analysis of PDE-5 expression in conditional medium of colon epithelium cell (n=3). (B, C) Matrigel-based invasion assay analysis of MDSC invasion under conditional medium stimulating (B). The number of MDSC per frame was calculated (C, n=3). (D, E) Matrigel-based invasion assay analysis of macrophage invasion under conditional medium stimulating (D). The number of macrophage per frame was calculated (E, n=3).
