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. 2017 Jan 1;7(1):77–87.

Figure 4.

Figure 4

Methylation analysis of VAV3 promoter in HaCaT cells. A: Schematic representation of the CpG island and CpG sites in the VAV3 promoter. For methylation analysis the VAV3 promoter was divided into 2 regions: R1 -599 to -307 with 34 CpGs and R2 -299 to +20 with 61 CpGs, the positions are relative to the transcription start site. The primers for MSP and BSP are indicated by black and red arrows, respectively. Each CpG site is represented by a vertical bar. B: The methylation status of the VAV3 promoter (R1 and R2) was determined by MSP in HaCaT cells with overexpression of DNMT3B and control HaCaT cells. U showed unmethylation-specific primer amplification, M showed methylation-specific primer amplification. C: BSP analysis of the VAV3 promoter (R1 and R2) in HaCaT cells with overexpression of DNMT3B and control HaCaT cells. Black circles represent methylated CpG site and white circles represent unmethylated CpG site. The red box shows the two regions more densely methylated by overexpression of DNMT3B.